Re: [DIYbio] Re: Can you freeze cellulolytic enzymes?

The use of glycerol is probably to prevent having to freeze/thaw enzymes
with each use; they can be used at -20 and immediately put back in
storage. So, I'd mimic this and use glycerol to keep enzymes happy.

Too much glycerol in the final reaction mix can mess with some enzymes
though, particularly DNA-modifying enzymes, so you may need to assess
how much you add and use the minimum needed to keep solutions liquid at
-20. I doubt this would have much bearing on cellulolytic enzymes, but
you never know!

On 30/01/13 16:20, jlund256 wrote:
> Enzymes are routinely stored in a 50% glycerol solution at -20C. They
> do not freeze because of the glycerol. Freeze / thaw would likely kill
> or seriously reduce enzyme activity. All these things are enzyme
> specific. Some enzymes don't like low temps or glycerol, but this is
> relatively rare. If these enzymes are sold commercially, check if
> anyone sells them as a glycerol stock.
>
> Jim Lund
>
>
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