Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

My results with the printer paper-

The dyes spread out and the paper looked kinda like a watercolor. No lines whatsoever. Printer paper doesn't work. That is what I concluded... maybe you guys can try it

On Saturday, January 19, 2013 4:11:43 PM UTC-8, Nathan McCorkle wrote:

So my thinking is everyone can get paper of some form, but not
agar/agarose... seems it's not ideal for DNA but it is for amino acids
and small proteins... it might be useful for DNA or large proteins in
certain assays though... i.e. yes/no assays or something

here are some things i dug up:

mentions a few paragraphs of history
https://www.idtdna.com/pages/docs/educational-resources/gel-electrophoresis.pdf

including
"
While paper and other solid support materials proved to be an
advantage over free
solutions for the electrophoretic analysis of biomolecules, gels were
adopted later
because gels not only minimized diffusion better than paper supports
they actually
participated in the separation process by interacting with the
migrating particles.
"

and referencing this article about Bothrops (pit viper snake) venom,
but it's in German (I think) and I can't read it (google translate
doesn't do much either because copy-paste don't keep the sepcial
characters)
http://nathanmccorkle.com/pdf/this%20might%20be%20about%20paper%20electrophoresis.pdf

I got thinking because I saw this chromatography paper for $0.65 per
foot and mentions "widely used for electrophoresis"
http://store01.prostores.com/servlet/thescienceshop/the-2012/CHROMATOGRAPHY-PAPER-2cm-Wide/Detail


thesis on
"Development of Paper electrophoresis technique for observation of
microgram quantities of protein" and "electrophoretic and
ultracentrifugal studies of soluble antigen-antibody complexes as a
method of determining antibody and antigen valences"
James T. Bradbury, California Institute of Technology, 1956
http://thesis.library.caltech.edu/995/1/Bradbury_jt_1956.pdf


--
-Nathan

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