Hi everyone,
I'm trying to design primers on my own. but now there are a few questions that Google couldn't answer me.
a) are primers single stranded DNA or double stranded?
All results from Google don't say anyhing about that. It's just like, everyone who writes thos pages knows, but it is too obvious to be mentioned.
I would strongly assume they are double stranded?
b)
I got Gene
ATG-AAA-NNNNNNNNNNNNNNNNNN-TTT-TAG
If double stranded Iwould just have to write down the sequence:
forward primer:
NNNrestriction - ATG - AAA
reverse primer:
TTT-TAG-NNNrestriction.site
Correct? (Not considering melting temps yet!)
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