My apologies if I'm rehashing old topics but I have a question and I can't seem to find the answer. I've heard various references to oligo synthesis without the use of an activator. Is this in fact possible? How feasible is it in terms of the quality of the oligos and the time necessary for the reaction? Secondly is there any waste product from reaction without an activator? For that matter with an activator aside from the activator it self? Thirdly is there any compelling reason why an activator and nucleotide can't be premixed. As I said forgive my ignorance of organic chemistry but I have an idea for something and I want to quickly get an idea of whether what I'm thinking of is plausible.
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