Alow me to consentrate your atention on the 3rd question. Is there any compelling reason not to premix the activator and nucleoside in an oligo synth cycle. Will there be undesirable side reactions if they are premixed? Also regarding the by products of activated coupling. Are they chemically inert wrt the other chemicals involved or can we expect side reactions if some are present in the premixed activator and nucliocide?
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[DIYbio] oligo synthesis without an activator?
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