[DIYbio] Re: How do you make and use acrylamide gels?

On Fri, Apr 1, 2011 at 2:41 PM, Dave Hosfield <hosfield@gmail.com> wrote:
> poor the resolving gel
> acrylamide solution into the gap between the taped plates. I usually
> did this with a pasteur pippette or a funnel constructed from a
> hypodermic needle that fits between the plates.. When the resolving
> gel is poured, use a pasteur pipette to add a thin layer of butanol to
> the top. The butanol is less dense than the acrylamide and ensures a
> uniform boundary between the resolving and stacking gel.

Thanks Dave!

I was reading on bio-rad.com about this other method of casting,
skipping the butanol. Have you experience with this type? Pros/cons?

"
Alternative Casting Procedure
It is possible to cast separation and stacking
gels one after another, with no intermediate
step requiring overlay solution (water-saturated
n-butanol). Recalculate your gel casting recipes
so that the separation gel solution contains 25%
(w/v) glycerol. Due to the significant difference
in density, the two solutions won't mix when the
stacking gel solution is carefully poured on top of
the resolving gel solution.
"


--
-Nathan

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