Re: [DIYbio] Effect of repeated autoclavation

From: Cathal Garvey <cathalgarvey@cathalgarvey.me>
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Sent: Wed, Feb 6, 2013 08:12:27 EST
Subject: Re: [DIYbio] Effect of repeated autoclavation

Had known that of sugars (although maillard is amino:sugar reaction, so
you can autoclave sugars separately and avoid this if a little bit of
caramelisation is OK), but not of glycerol.

Autoclaving: the quick and dirty sterilisation solution. :)

On 06/02/13 13:07, Matt Lawes wrote:
> Yep the effect on sugars (heat hydrolysis) and Millard reaction
> (primarily with peptides and amino acids) of autoclaving - especially
> repeated autoclaving. I've always made concentrated sugar solutions,
> filter sterilized, and aseptically added to media after autoclaving.
>
>>matt
>
> /Sent from my Verizon Wireless 4G LTE DROID/
>
>
> -----Original message-----
>
>     *From: *Cathal Garvey <cathalgarvey@cathalgarvey.me>*
>     To: *"diybio@googlegroups.com" <diybio@googlegroups.com>*
>     Sent: *Wed, Feb 6, 2013 06:11:31 EST*
>     Subject: *Re: [DIYbio] Effect of repeated autoclavation
>
>     Good to know, I had never heard that! Pretty relevant to luminescence
>     broth if anyone's doing bioluminescent bacterial isolations.
>
>     On 06/02/13 05:49, Robert Sidney Cox III wrote:
>     > Glycerol will create Glyceraldehyde, which is toxic
>     >
>     > On Wednesday, February 6, 2013 4:02:25 AM UTC+9, Cathal Garvey wrote:
>     >
>     >     For LB, this isn't so much of an issue, but for media that contain
>     >     sugars you can get caramelisation of the sugar, splitting of
>     >     disaccharides, or maillard reactions, all of which might
>     interfere with
>     >     some sensitive experiments, bacteria, or what have you.
>     >
>     >     For most experiments though, it won't make much of a difference
>     >     provided
>     >     you try to use the same media batch for everything; doing
>     otherwise,
>     >     you
>     >     might have trouble telling what's different between samples;
>     whether to
>     >     blame the bugs or the broth.
>     >
>     >     On 05/02/13 18:54, Avery louie wrote:
>     >     > It can mess up the concentration by removing water, which
>     makes it
>     >     > darker as has been mentioned.
>     >     >
>     >     > --A
>     >     >
>     >     > On Tue, Feb 5, 2013 at 1:30 PM, Daniel C. <dcroo...@gmail.com
>     >     <javascript:>
>     >     > <mailto:dcroo...@gmail.com <javascript:>>> wrote:
>     >     >
>     >     >     On Tue, Feb 5, 2013 at 12:01 PM, jarlemag
>     <jarle...@gmail.com
>     >     <javascript:>
>     >     >     <mailto:jarle...@gmail.com <javascript:>>> wrote:
>     >     >     > Hello. A simple question popped up in the lab today, and I
>     >     thought
>     >     >     perhaps
>     >     >     > some of you would know: Will autoclaving the same medium
>     >     multiple
>     >     >     times
>     >     >     > degrade the quality of common complex growth media
>     such as LB?
>     >     >
>     >     >     I can't speak to the effects on LB, but repeatedly
>     autoclaving
>     >     growth
>     >     >     medium for Saccharomyces cerevisiae (yeast) can actually
>     >     improve it by
>     >     >     causing precipitation of chemicals that would otherwise form
>     >     >     particulates and cloud up your final product.  Because the
>     >     important
>     >     >     chemicals are essentially sugar, autoclaving (even multiple
>     >     times)
>     >     >     won't make the medium less suitable but it can make it
>     >     progressively
>     >     >     darker in color.
>     >     >
>     >     >     -Dan
>     >     >
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