Re: [DIYbio] Effect of repeated autoclavation

Had known that of sugars (although maillard is amino:sugar reaction, so
you can autoclave sugars separately and avoid this if a little bit of
caramelisation is OK), but not of glycerol.

Autoclaving: the quick and dirty sterilisation solution. :)

On 06/02/13 13:07, Matt Lawes wrote:
> Yep the effect on sugars (heat hydrolysis) and Millard reaction
> (primarily with peptides and amino acids) of autoclaving - especially
> repeated autoclaving. I've always made concentrated sugar solutions,
> filter sterilized, and aseptically added to media after autoclaving.
>
>>matt
>
> /Sent from my Verizon Wireless 4G LTE DROID/
>
>
> -----Original message-----
>
> *From: *Cathal Garvey <cathalgarvey@cathalgarvey.me>*
> To: *"diybio@googlegroups.com" <diybio@googlegroups.com>*
> Sent: *Wed, Feb 6, 2013 06:11:31 EST*
> Subject: *Re: [DIYbio] Effect of repeated autoclavation
>
> Good to know, I had never heard that! Pretty relevant to luminescence
> broth if anyone's doing bioluminescent bacterial isolations.
>
> On 06/02/13 05:49, Robert Sidney Cox III wrote:
> > Glycerol will create Glyceraldehyde, which is toxic
> >
> > On Wednesday, February 6, 2013 4:02:25 AM UTC+9, Cathal Garvey wrote:
> >
> > For LB, this isn't so much of an issue, but for media that contain
> > sugars you can get caramelisation of the sugar, splitting of
> > disaccharides, or maillard reactions, all of which might
> interfere with
> > some sensitive experiments, bacteria, or what have you.
> >
> > For most experiments though, it won't make much of a difference
> > provided
> > you try to use the same media batch for everything; doing
> otherwise,
> > you
> > might have trouble telling what's different between samples;
> whether to
> > blame the bugs or the broth.
> >
> > On 05/02/13 18:54, Avery louie wrote:
> > > It can mess up the concentration by removing water, which
> makes it
> > > darker as has been mentioned.
> > >
> > > --A
> > >
> > > On Tue, Feb 5, 2013 at 1:30 PM, Daniel C. <dcroo...@gmail.com
> > <javascript:>
> > > <mailto:dcroo...@gmail.com <javascript:>>> wrote:
> > >
> > > On Tue, Feb 5, 2013 at 12:01 PM, jarlemag
> <jarle...@gmail.com
> > <javascript:>
> > > <mailto:jarle...@gmail.com <javascript:>>> wrote:
> > > > Hello. A simple question popped up in the lab today, and I
> > thought
> > > perhaps
> > > > some of you would know: Will autoclaving the same medium
> > multiple
> > > times
> > > > degrade the quality of common complex growth media
> such as LB?
> > >
> > > I can't speak to the effects on LB, but repeatedly
> autoclaving
> > growth
> > > medium for Saccharomyces cerevisiae (yeast) can actually
> > improve it by
> > > causing precipitation of chemicals that would otherwise form
> > > particulates and cloud up your final product. Because the
> > important
> > > chemicals are essentially sugar, autoclaving (even multiple
> > times)
> > > won't make the medium less suitable but it can make it
> > progressively
> > > darker in color.
> > >
> > > -Dan
> > >
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