Yep the effect on sugars (heat hydrolysis) and Millard reaction (primarily with peptides and amino acids) of autoclaving - especially repeated autoclaving. I've always made concentrated sugar solutions, filter sterilized, and aseptically added to media after autoclaving.
>matt
Sent from my Verizon Wireless 4G LTE DROID
>matt
Sent from my Verizon Wireless 4G LTE DROID
-----Original message-----
From: Cathal Garvey <cathalgarvey@cathalgarvey.me>
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Sent: Wed, Feb 6, 2013 06:11:31 EST
Subject: Re: [DIYbio] Effect of repeated autoclavationGood to know, I had never heard that! Pretty relevant to luminescence
broth if anyone's doing bioluminescent bacterial isolations.
On 06/02/13 05:49, Robert Sidney Cox III wrote:
> Glycerol will create Glyceraldehyde, which is toxic
>
> On Wednesday, February 6, 2013 4:02:25 AM UTC+9, Cathal Garvey wrote:
>
> For LB, this isn't so much of an issue, but for media that contain
> sugars you can get caramelisation of the sugar, splitting of
> disaccharides, or maillard reactions, all of which might interfere with
> some sensitive experiments, bacteria, or what have you.
>
> For most experiments though, it won't make much of a difference
> provided
> you try to use the same media batch for everything; doing otherwise,
> you
> might have trouble telling what's different between samples; whether to
> blame the bugs or the broth.
>
> On 05/02/13 18:54, Avery louie wrote:
> > It can mess up the concentration by removing water, which makes it
> > darker as has been mentioned.
> >
> > --A
> >
> > On Tue, Feb 5, 2013 at 1:30 PM, Daniel C. <dcroo...@gmail.com
> <javascript:>
> > <mailto:dcroo...@gmail.com <javascript:>>> wrote:
> >
> > On Tue, Feb 5, 2013 at 12:01 PM, jarlemag <jarle...@gmail.com
> <javascript:>
> > <mailto:jarle...@gmail.com <javascript:>>> wrote:
> > > Hello. A simple question popped up in the lab today, and I
> thought
> > perhaps
> > > some of you would know: Will autoclaving the same medium
> multiple
> > times
> > > degrade the quality of common complex growth media such as LB?
> >
> > I can't speak to the effects on LB, but repeatedly autoclaving
> growth
> > medium for Saccharomyces cerevisiae (yeast) can actually
> improve it by
> > causing precipitation of chemicals that would otherwise form
> > particulates and cloud up your final product. Because the
> important
> > chemicals are essentially sugar, autoclaving (even multiple
> times)
> > won't make the medium less suitable but it can make it
> progressively
> > darker in color.
> >
> > -Dan
> >
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