The Activator protonates the amine on the phosphoramidite so that it attacks the 5' hydroxyl and thus coupling occurs.
So, you need both TOGETHER for nucleoside activation and thus coupling....
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-------- Original message --------
From: peter clark <petergrahamclark@blueyonder.co.uk>
Date: 02/02/2013 11:27 AM (GMT-08:00)
To: diybio@googlegroups.com
Subject: Re: [DIYbio] oligo synthesis without an activator?
I thought the activator functioned in combination with the nucleoside
and would not function applied one after the other with a wash in
between?
On 2 Feb 2013, at 16:53, Cathal Garvey wrote:
> I don't know much about this besides what's available on wikipedia,
> but
> my understanding is that you keep the activator separate from the next
> nucleoside so you can wash away nucleosides before activation of the
> next stage.
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