Re: [DIYbio] DIY Microfluidics + Parasitology Diagnostics

On Sat, Feb 2, 2013 at 8:18 PM, Gene Hacker <cam626@gmail.com> wrote:
> Looks like you have sufficient resolution to build a gene synthesizer.

You can synthesize genes in a test tube, so resolution isn't the
limit. The idea is getting small enough reactions such that you save
on reagents, but you need to also produce enough DNA such that you can
do PCR or a transformation at the end.


> Problem is, the chemicals involved in DNA synthesis might do a number on
> PDMS based devices.

There are several papers showing alternative synthesis chemistries to
get around PDMS swelling, which is mainly a problem with
organic/hydrophobic solvents.

> So you'll have to use something more solvent resistant
> like the elastomer PFPE, which happens to be processed in a similar manner.
> Though you might need an inert atmosphere to fabricate them though.

I don't know about PFPE processing, but you also need inert atmosphere
during synthesis, and you need everything to be dry from water or your
coupling efficiency will be horrible (I believe it essentially
competes with the 5' OH, since they both look the same to the
phosphoramidite ).

>
>
> On a lighter note, you also have sufficient resolution to make the sound
> amplification chip for this totally steampunk fluidic gramophone

Link to that? Sounds like it might sound cool!

--
-Nathan

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