Hi all,
Has anyone experience, how big a plasmid can get, so that you still can insert it into E.Coli / Agrobacterium using PEG transformation? Or is this no problem anyway when supercoiled?
When using electroporation, can the plasmid be bigger?
Are up to 16 kbp ok?
Is there recombination problems with E.Coli? (If no genomic DNA of E Coli is in the plasmid)
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