Think of the actual diameter as a function of increasing circumference. I used 20k+ plasmids routinely. There is not much change in its size. Supercoiled is just long but only a few angstroms wide. No biggie. Let me know if you need peg help. We can tandem experiment if you need concurrent measurements/observations.
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
Hi all,
Has anyone experience, how big a plasmid can get, so that you still can insert it into E.Coli / Agrobacterium using PEG transformation? Or is this no problem anyway when supercoiled?
When using electroporation, can the plasmid be bigger?
Are up to 16 kbp ok?
Is there recombination problems with E.Coli? (If no genomic DNA of E Coli is in the plasmid)
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