My thinking is in the case where the dna is partially bound and is being pulled via its charge across the strip. Could the TAE cause binding? If it does not then it should be fine like you mentioned. The G force of a centrifuge is, i think, much higher than the pull of DNA towards the positive pole so it wont matter. I'm not sure why I thought it would be torn up. Could you think of anything going wrong with using silica in this scenario?
On Friday, February 1, 2013, Nathan McCorkle wrote:
On Fri, Feb 1, 2013 at 3:55 PM, Sebastian S. Cocioba <scocioba@gmail.com> wrote:--
> If given the proper conditions the DNA would bind and be stuck. As it travels it may shear. Since electrophoresis applies a constant force onto the DNA, it seems that it would pull it to shreds. I believe someone mentioned this exact idea in a previous post.
Wait, why would it shred? I used to use silica spin-columns, they
didn't shred the DNA, though varied salt and pH buffers allow
selective binding/unbinding to allow purification of certain size DNAs
--
-Nathan
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