On Thursday, February 28, 2013 1:53:37 PM UTC-8, Nathan McCorkle wrote:
Cool! My thinking is throw the reversible terminators in a pipeline
with terminal transferase, and voila, green chemistry DNA synthesis
(well depending on the reversible terminators!)
That is precisely how a lot of DNA synthesis works. It's also how a lot of microarrays get synthesized, by using light-directed synthesis directly on the chip. In fact, there are strong links between easily/reliably achievable read lengths in DNA sequencing, oligo lengths in DNA synthesis, and probe lengths in microarrays.
Most of the current NGS methods are also called "sequencing-by-synthesis" precisely for this reason. A counter example would nanopore sequencing, where the DNA is read by pulling it through a pore, rather than by synthesizing a complementary strand one base at a time.
Patrik
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