Regarding reactants/tutorials, openbiotech.com has MasterMix to use. It looks like they even sell the kit itself.
There are many protocols for PCR online, and if you have problems feel free to post to the group and someone can help you out. One advantage of the OpenPCR machine is that you know people on the diybio group have them and can help troubleshoot. Its a lot harder to troubleshoot a random ebay device that wasn't designed for easy repair.
On the subject of costs, I'd argue that reactants and plasmids are expensive enough that the x00 dollars you might save on a new device will be outweighed by a few faulty reactions.
On Friday, February 1, 2013 1:27:20 PM UTC-5, Josiah Zayner wrote:
You are buying a product from the eBay sellers, not directly supporting them, you are just paying the person who is charging the least.--
If someone is willing to pay 5x what they could pay there usually is a reason. i.e. why someone would buy an OpenPCR machine rather than a PCR machine off of eBay. If someone buys an OpenPCR machine they are either trying to support OpenPCR, having a need, like needing something shipped outside the US, or just not trusting eBay.
I am not arguing that there are not people from outside the US in DIYBio or on this list.
Cathal, I have no idea what Josh and Tito are about, this is true.On Fri, Feb 1, 2013 at 12:14 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
On Thu, Jan 31, 2013 at 3:32 PM, Josiah Zayner <josiah...@gmail.com> wrote:That's definitely what the eBay sellers are in it for, so why are you
> Seriously, don't support people who are just out to make
> money off of DiyBio folks.
supporting their marketplace? openPCR will survive if it can compete,
the ebay links enable potential consumers to be informed of the
market, but c'mon the openPCR guys started this as a DIY project like
all the rest we talk about in this group.
--
-Nathan
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