Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

On Wed, Jan 30, 2013 at 7:36 AM, John Griessen <john@industromatic.com> wrote:
> On 01/30/2013 07:48 AM, Dakota Hamill wrote:
>>
>> Trying to think of other ways to seal off a narrow channel, to control the
>> initial dispersion as you said, but keep open a running
>> lane for the DNA or dye to migrate.
>
>
> It seems to me that ordinary filter papers wick rapidly, making wide blurs
> out of any sample put on them.
>
> If you coated filter paper with agarose then dried it be convenient paper
> again, it might
> perform more like agarose -- low dispersion. If drying somehow ruins
> it,then store
> it wet refrigerated/frozen and you'd get the agarose narrow dispersion
> benefits with a small amount and the convenience of paper.
>
> It might pay to try different papers. Clay coated papers for photo printing
> might be good.
> Reading about TLC made me try searching for silica gel paper and it exists:
> http://www.fishersci.com/ecomm/servlet/fsproductdetail_10652_785049__-1_0
> and is thick, which is good for electrophoresis.
>

I just so happen to have about a gallon of silica powder with a 254nm
excited UV fluorophore (so your compound creates a dark spot) and some
filter paper... I don't have any ladder though, and only methylene
blue stain right now.

https://picasaweb.google.com/lh/photo/ZoqH0q56h_bFbO-lXVqVF-1Smq-NzwwjGgUaj45-NKQ?feat=directlink


--
-Nathan

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