You can start a Kickstarter for basically anything. That is your choice.
Here are the questions:
What skills and knowledge do you possess that would make people believe that you could accomplish one of the projects you propose?
Have you done literature searches? Environmental sampling and identifying species is constantly being done. What are you doing different and what will you add?
What part of the 16S do you plan to sequence? In which way do you plan to sequence this(through PCR products? cloning?)?
16S is prokaryotic what do you plan on sequencing for meats?
What would this add the the general knowledge of science or the DIYBio community?
Your budget seems low. For $300 - $500 I doubt you could culture and sequence more than a handful of bacterial strains probably very few if you have never done it before.
If someone had a very well thought out and planned research proposal and the skills to accomplish it. I think the DIYBio community would be supportive. As to how supportive I do not know.
On Thursday, March 21, 2013 9:02:57 AM UTC-5, jarlemag wrote:
Hi. For a while now, I've been thinking of a couple of molecular biology hobby projects regarding species identification of both microbial samples and consumer meat I would like to try.--
However, the costs for primer synthesis and sequencing reactions can be, while not too bad, a bit of a strain on a student budget.
So, do you think there would be a willingness or interest in the DIY community to crowd-fund small investigative projects? Already owning basic equipment, as I do, I figure that for a couple hundred $/€, one can do a fair bit of interesting stuff. I imagine about $300-$500 is a reasonable cost range for a small project, but I might of course cover parts of the costs myself, thus decreasing the funding goal for contributions.
Possible projcects I have in mind are:
*Culturing environmental microbial followed by 16S RNA analysis. What (culturable) organisms live in different places?
*Testing and evaluating PCR methods for DIY species identification of meat. (this was discussed on the mailing list a little while ago). Could also include 16S RNA sequencing.
What do you think? Is it worth trying to start a KickStarter for something like this? It would of course have more details than the above descriptions, with article references and an experimental plan to be followed. Results would be made available on OpenWetware
Best regards,
Jarle Pahr
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