miRNAs are RNAs so primer synthesis is a no go because that is DNA. You can perhaps reverse transcribe a DNA element and then purify it and then inject/transfect it into a cell but this seems beyond DIYBio at the moment.
miRNAs go through extensive processing in the cell so I think most people tend to use shRNA and other things.
miRNA expression
http://www.origene.com/MicroRNA/
shRNA expression
http://www.genscript.com/siRNA_service.html?gclid=CLv6sZqdjrYCFYFDMgod-lMAEA
On Sunday, March 17, 2013 8:17:16 AM UTC-5, Mega wrote:
Hi,--
although I don't have any projects at the moment in this direction, I'm quite interested in miRNAs...
Can one design a mRNA like this:
If you have a promoter, there's the transcription initiation site where the promoter ends. Next to it you wouldn't attach any ribosome binding sites (because all you are interested in is the RNA, protein synthesis would be waste of energy). Then I think you would add the complementary sequence of the CDS (the first 20 nucleotides or so), excluding the ATG so it won't have a chance to silence other proteins.
Should work like this, right?
What makes it specifically interesting is that they are so short you basically could have them synthesized as primers, in this size without expensive purification...
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