I haven't seen the exact protocols used for this - and I doubt they did much in terms of optimization for this particular ultrasonic cleaner. But definitely a clear proof of principle...
Patrik
On Tuesday, February 26, 2013 2:59:15 AM UTC-8, Patrik D'haeseleer wrote:
Alright - sounds like we're going to give this a go at BioCurious this week, using the 35W model I got. We'll let you know how it works out...--
Patrik
On Monday, February 25, 2013 2:37:30 AM UTC-8, Cathal Garvey wrote:-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA256
Does the paper include information on frequency and wattage? I've not
yet tried sonoporation but I'd love to see it tested using
commercially available jewellery cleaners, because there's a lot of
"who knows?" around the issue. If the frequencies are significantly
different, it:
A) Mightn't work at all
B) Might need more or less time to work
C) Might have a different spectrum of useless/useful/murderous activity.
Give it a go! :)
On 02/25/2013 07:27 AM, Patrik D'haeseleer wrote:
>
>
> On Sunday, February 24, 2013 3:56:56 AM UTC-8, Mega wrote:
>
> There once was a sonic cleaner in the store for some 20�.
>
>
> I thought about buying it, but won't it damage the plasmids while
> transforming?????
>
>
> "Ultrasound treatment for 5�60 s results in plasmid transfer to
> /P. putida/ UWC1 (/P/ < 0.05). Exposure time of 10 s gives the
> highest transfer efficiency. No plasmid transfer occurred in the
> absence of ultrasound treatment. (B) Ultrasound reduced /P. putida/
> UWC1 survival following 60 s exposure time, but shorter treatments
> had little effect on bacterial survival (/P/ < 0.05). (C) Plasmid
> DNA was prone to cleavage following ultrasound exposure times
> longer than 30 s"
>
> So yes, you can definitely damage the plasmids and the cells
> themselves with longer exposure times. But you only need a 10s
> exposure to get optimal DNA transfer.
>
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- --
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