Re: [DIYbio] Re: Do enzymes really need to be "frozen"?

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I'm not sure, but I don't think most restriction enzymes need ATP,
though many other types of enzyme do.

On 03/24/2013 11:36 AM, Mega wrote:
>
> Have to revive this old discussion...
>
>
> "This paper claims that all the restriction enzymes they tested
> maintained their activity at room temperature for a week, and most
> maintained activity for a full year"
>
> I always thought (and heard from a lab worker) that restriction
> enzymes need ATP and therefore the entire solution has to be kept
> at -20°C to prevent ATP decay... But didn't read that they added
> ATP then? Is the ATP story wrong?
>
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