Re: [DIYbio] Re: Do enzymes really need to be "frozen"?

Can anyone list some enzymes that need to be at -20?
Ones that we need to worry about if the power goes out for a couple of days.

On Sun, Mar 24, 2013 at 7:55 AM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
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> I'm not sure, but I don't think most restriction enzymes need ATP,
> though many other types of enzyme do.
>
> On 03/24/2013 11:36 AM, Mega wrote:
>>
>> Have to revive this old discussion...
>>
>>
>> "This paper claims that all the restriction enzymes they tested
>> maintained their activity at room temperature for a week, and most
>> maintained activity for a full year"
>>
>> I always thought (and heard from a lab worker) that restriction
>> enzymes need ATP and therefore the entire solution has to be kept
>> at -20°C to prevent ATP decay... But didn't read that they added
>> ATP then? Is the ATP story wrong?

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