Really? But what powers them then? just the energy from room temperature?
So, basicallly EcoRI and HindIII are very stable it seems.
1. For EcoRI, no
appreciable decrease in activity was
noted until eight weeks after transfer to
37°C, and some activity still remained
after 12 weeks (the absence of activity
of the 4°C sample after two weeks is
most likely due to experimental error
because subsequent reactions with the
same enzyme exhibited activity).
HindIII appeared to be even more stable, and it was not until six months after transfer to 37°C that a reduction in
activity could be seen. For SmaI, although no activity was noted after storage for one week at 37°C, this is again
likely to be due to experimental error
because a similar result was observed
with the -20°C sample for digestion at
this time point. Reduced activity was
subsequently noted after two and four
weeks of incubation at 37°C, although
none remained after eight weeks.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/FYWpzvTA5n0J.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment