There are three different types of restriction enzymes. Type I cuts DNA at random locations as far as 1000 or more base-pairs from the recognition site. Type III cuts at approximately 25 base-pairs from the site. Types I and III require ATP and may be large enzymes with multiple subunits. Type II enzymes, which are predominantly used in biotechnology, cut DNA within the recognized sequence without the need for ATP, and are smaller and simpler.
So no ATP for molecular cloning restriction enzymes!!
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