Could be that I get a transposon soon. That can integrate into any bacteria.
But if you need it urgently, why not do a PCR of an unneccesary E.Coli gene, disrupt it in the midlle, insert a Kanamycin resistance and the gene of interest.
So you get a "linear plasmid which can't reproduce" in E.Coli. To inherit the gene, bacteria have to insert it into their chromosomes via homologous recombination.
So all colonies will then have taken up the DNA.
-- -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/6U7Kt5oRh1cJ.
For more options, visit https://groups.google.com/groups/opt_out.






0 comments:
Post a Comment