I would like to know when you finish that transposon, I am thinking about inserting pOpenTaq into some E coli :)
On Apr 7, 2013, at 10:55 AM, Mega <masterstorm123@gmail.com> wrote:
Could be that I get a transposon soon. That can integrate into any bacteria.But if you need it urgently, why not do a PCR of an unneccesary E.Coli gene, disrupt it in the midlle, insert a Kanamycin resistance and the gene of interest.So you get a "linear plasmid which can't reproduce" in E.Coli. To inherit the gene, bacteria have to insert it into their chromosomes via homologous recombination.So all colonies will then have taken up the DNA.--
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