Re: [DIYbio] Easiest transformation

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Did they mention whether this works with any strain, or some
particularly domesticated strains in their labs?

Cool to know this is a "routine" hack, though! I do love timesaving
shortcuts. :)

On 04/02/2013 02:17 PM, Mega wrote:
> Good news, everyone.
>
> A proffessor of mine told me that some microbiologists were
> laughing at them because they used competent e.coli.
>
> Those microbiologists just incubated the colis with the plasmid for
> an half hour and then selected. There always were some colonies...
>
>
> of course, this won't work when doing ligation, but for amplyfying
> a plasmid it is said to do its job...
>

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