Both are optional.
Freeze the cells to do the lysis.
Breakdown the proteins with bromelain or meat tenderizer.
Mac Cowell did it with detergent and salt.
Patrik D'haeseleer did this when we were at Science Hack Day in 2011 but without the detergent and salt. On Tue, May 7, 2013 at 5:02 PM, Dakota Hamill <dkotes@gmail.com> wrote:
But if you are making a drinkable shot how do you get around a lysis buffer and salt?--
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