http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-61779-558-9_35
Sometimes in a paper, the authors email is given. You could then email them and kindly ask for a plasmid. They will ask you for your university affiliation. Also make sure that you offer them a fee for handling and shipment and for their efforts.
On the other hand, if you can do PCR, you could also just amplify a chloroplast DNA fragment (see in the literature, there was one that naturally contains a MunI restriction site).
Btw, my hybrid promoter will also express in E.Coli, so at least you get glowing bugs :D But could have done that with pVIB too, just cheaper :D
-- Sometimes in a paper, the authors email is given. You could then email them and kindly ask for a plasmid. They will ask you for your university affiliation. Also make sure that you offer them a fee for handling and shipment and for their efforts.
On the other hand, if you can do PCR, you could also just amplify a chloroplast DNA fragment (see in the literature, there was one that naturally contains a MunI restriction site).
Btw, my hybrid promoter will also express in E.Coli, so at least you get glowing bugs :D But could have done that with pVIB too, just cheaper :D
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/283bf402-91cc-4fb0-a289-525256a28057%40googlegroups.com.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment