On Tuesday, October 15, 2013 11:16:02 AM UTC-7, Mackenzie Cowell wrote:
I would assume uBiome is using iTag sequencing, multiplexing a couple hundred samples on a single Illumina run. There's no way they can get down to $69 for a mixed sample without doing next-gen.
Of course, uBiome's $69 offering is for 16S - they'd have to target 18S or ITS for fungi. Not sure how much optimization that would require beyond merely swapping out a different set of primers.
Patrik
-- uBiome is offering some kind of barcoding service on scienceexchange for $69, but I don't think it's next-gen based. But they might be open to an affordable collaboration. http://widgets.scienceexchange.com/ facilities/ubiome/services/ 16s-rdna-sequencing-analysis
I would assume uBiome is using iTag sequencing, multiplexing a couple hundred samples on a single Illumina run. There's no way they can get down to $69 for a mixed sample without doing next-gen.
Of course, uBiome's $69 offering is for 16S - they'd have to target 18S or ITS for fungi. Not sure how much optimization that would require beyond merely swapping out a different set of primers.
Patrik
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