Re: [DIYbio] Protein Purification

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Yea, ELPs: under the right salt/temperature conditions, your protein
alone precipitates, and can then usually re-dissolve once purified by a
quick spin cycle. They're great, really trendy, and patented to the
hilt AFAIK.

My design motivations are:
1) Triviality
2) Ubiquity
3) Freedom

So a purification method must be cheap or use ubiquitous tools, resins
or whatever, really easy, and non-patented.

Sadly, almost everything is patented. I hit upon a rare thing that
wasn't patented, and I'm running with it. Of course, some asshat might
sue me anyway because IP or something, but at least I tried; the point
is more to encourage others to work with what I've built and make
better things, help create an ecosystem. You can't do that with a
patented foundation like ELPs. :-/

On Tue, 15 Oct 2013 11:19:48 -0700
Mac Cowell <mac@diybio.org> wrote:

> The CU-Boulder iGEM 2013 team focused on developing diy methods for
> molecular biology. miniprep column regeneration, gel electrophoresis
> band extraction, Taq cloning, and three methods for protein
> purification. One of these latter methods is based on adding a
> specific repeat motif to your protein of interest that will cause it
> to precipitate in the presence of calcium. Not sure if the motif effs
> up the activity of the protein (very possible), but it's an
> interesting start.
>
> http://2013.igem.org/Team:CU-Boulder/Project/Kit/Purification
>
> Mac
>
>
> On Sun, Oct 13, 2013 at 5:21 PM, Cathal Garvey
> <cathalgarvey@cathalgarvey.me
> > wrote:
>
> > Hey Michael! Glad you enjoyed the podcast. :)
> >
> > I'm still working on it, and if everything goes well and I'm lucky,
> > I ought to have something soon. Indeed, if it works, my next round
> > of gene synthesis will be to order a set of enzymes for easy
> > purification, to sell as kits at a reasonable price, with full
> > "sourcecode" included and no nonsense like Patents!
> >
> > Right now, we're stuck with only one or two proteins commonly
> > purified DIY; thermostable polymerases. That's because they can be
> > prepared by freezing and boiling cell samples, and using the clear
> > liquid after filtering or sedimenting cell matter. It's nice that
> > polymerases are that easy, but we're lagging everywhere else,
> > because purification systems either suck (expense, difficulty,
> > secret-sauce) or are patented to the hilt. Hopefully I can change
> > that, soon.
> >
> > As far as Sydney labspace, I'm not sure! Hopefully someone here will
> > chip in?
> >
> > Feel free to call on me for a tour of my very small lab if you're
> > ever in Cork. ;)
> >
> > On Sun, 13 Oct 2013 02:31:57 -0700 (PDT)
> > Michael Brown <c2104421@hotmail.com> wrote:
> >
> > > Hello,
> > > I've only just become aware of DIYBio, and have recently
> > > completed listening to a Podcast; SynBioBeta Podcast #4: DIYBio.
> > > In it, an individual from Cork named Cathal Garvey was trying to
> > > make a recombinant protein purification system within the reach
> > > of a layperson. I wish to enquire as to his progress, and would
> > > also like to know about the Hackerspace in sydney, as I am an
> > > unemployed biotechnologist looking to utilise some lab space as a
> > > Hobby.
> > >
> >
> >
>