I may be able to get a ready-to use fluorescent protein yeast integrative plasmid, rather than need to cut GFP into pKlac2.
However, the ready-to -use plasmid often either contain
a) URA marker, so you need a URA-deficient strain of backers yeast -> cost, time, where to get it from?
b) antibiotic markers - chloramphenicol, kanamycin, etc.
Although I always tell everyone I know that it is very unlikely that the antibiotic gene gets into your gut bacteria, and chances are even smaller that they can even express it... I would have a bad gut feeling of eating yeast with a resistance gene... Is it just superstitious me, or is there some truth in it?
The yeast DNA will survive baking very well, so in theory it should be possible that bacteria take up a fragment. But without a SD-type RBS...?
Would you eat it?
-- However, the ready-to -use plasmid often either contain
a) URA marker, so you need a URA-deficient strain of backers yeast -> cost, time, where to get it from?
b) antibiotic markers - chloramphenicol, kanamycin, etc.
Although I always tell everyone I know that it is very unlikely that the antibiotic gene gets into your gut bacteria, and chances are even smaller that they can even express it... I would have a bad gut feeling of eating yeast with a resistance gene... Is it just superstitious me, or is there some truth in it?
The yeast DNA will survive baking very well, so in theory it should be possible that bacteria take up a fragment. But without a SD-type RBS...?
Would you eat it?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/b48d5bb8-1314-415d-a7a4-ceb9d6386b33%40googlegroups.com.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment