Re: [DIYbio] Re: Small side project - baking GFP bread - exhibtion

Horizontal transfer exists, maybe search for GI (gastrointestinal
tract) species horizontal transfer?

I wouldn't worry about antibiotics not commonly used (or not approved)
in humans, though they might still be used as last-resort measure for
really sick people... it probably doesn't happen much because you'd
probably have to be in really really bad shape to get your doctor to
give you unapproved/blacklisted medication. That said, you should
avoid non-approved drugs that might impact the pressure on some of the
approved-for-humans antibiotic mechanisms.

On Mon, Nov 11, 2013 at 12:03 PM, Mega [Andreas Stuermer]
<masterstorm123@gmail.com> wrote:
> I may be able to get a ready-to use fluorescent protein yeast integrative
> plasmid, rather than need to cut GFP into pKlac2.
>
> However, the ready-to -use plasmid often either contain
>
> a) URA marker, so you need a URA-deficient strain of backers yeast -> cost,
> time, where to get it from?
> b) antibiotic markers - chloramphenicol, kanamycin, etc.
>
>
> Although I always tell everyone I know that it is very unlikely that the
> antibiotic gene gets into your gut bacteria, and chances are even smaller
> that they can even express it... I would have a bad gut feeling of eating
> yeast with a resistance gene... Is it just superstitious me, or is there
> some truth in it?
>
> The yeast DNA will survive baking very well, so in theory it should be
> possible that bacteria take up a fragment. But without a SD-type RBS...?
>
>
> Would you eat it?
>
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--
-Nathan

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