Re: [DIYbio] DIY Microfluidics Device Manufacturing

I'm building a gear stepper motor controlled 3-point kinematic mount
now for focusing a projected image onto a plane. I've got a DMD/DLP
projector with the focus ring turned past the factory set-screw
projecting 1024 pixels across ~1cm. Its aslso rigged up to a
microcontroller so it thinks the fans are on and running properly
(since I took out the lamp, there are no fans). Now I'm working on
stepper microstepping with an arduino, but can't do any wet-lab until
next month. I got some eBay photoresist for PCBs and got a local
photoresist manufacturer to do some simulations for me to get the
right curved channels with their product. Another thing I'll try is
the dry photoresist films they sell for PCBs, just heard today that
they're likely 50-100 microns thick. Getting curved channels often
requires defocused exposure and some combination of lower-temp/longer
post exposure bake and/or incomplete develepment.

You might also try an 8000 DPI mask from
http://www.laserlab.com/photoplot.php instead of a digital projector
mask, they use a CNC laser to expose the photo-film. Then you could
try contact exposure of the eBay dry films, and use eBay developer
too. Then you'd have your pattern in the film, and could put a glass
slide on top and try it out. If you started with the film on a
copper-clad PCB you could etch a circuit board, or use the etched
copper board (clean off the film first) as a master stamp for pressing
into uncured silicone. Peel off the silicon layer and sandwich between
2 glass slides.

You can also use double-sided sticky tape, a laser cutter, and some
plastic or glass slides. Tape is around 30 or 40 microns thick I
believe, normal stuff works, or they sell engineering-grade tapes with
more chemically resistant/compatible adhesives or bulk plastic.

Often oxygen plasma is used to bond glass and silicones, you can make
a plasma bonder easily with a hand vacuum pump, an oxygen cylinder, a
home food microwave, and some HomeDepot/hardware-store PVC pipe and
rubber gasket sheeting:
Homemade Oxygen Plasma Etcher & PDMS to Glass Bonding Test - Black Box Labs
http://www.youtube.com/watch?v=N-R0_nXpc7I

If you're talking software, get AutoCAD, inventor doesn't work well
with Microns as units... with AutoCAD comes this, though it could
probably be hacked into something open-source:
http://groups.csail.mit.edu/cag/micado/


On Thu, Nov 21, 2013 at 3:02 PM, <scocioba@gmail.com> wrote:
>
> You guys and gals know of anyone working on micro-cnc designs or
> Microfluidics channel layout making protocols. Something like resin casting,
> cnc milling, etc. I had a project on the backburner and wanted to give some
> chemostat-esque apparatuses (aparati?) a shot. Just got a hankering for
> making tiny channels in plastic and playing with chemostats. Thanks!
> Sent from Windows Mail
>
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--
-Nathan

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