You can't put a filter between the coverslip and the objective. Microscope objectives are make for particular cover slip thicknesses (that thickness is usually printed under the magnification number on the objective). The thickness of the coverslip changes the optics, so the objectives are made to accomodate that.
I have never heard of UV blocking cover slips. But it is easy to put filters in the light path above the objective, such as at the bottom of the eyepiece, or in front of the camera. I often use my SLR without a lens, so the only thing between the camera sensor and the subject is the objective lens. That leaves a lot of room for putting in filters (and you get a much better camera than the ones they ship with microscopes).
On Sun, Dec 8, 2013 at 11:08 AM, Matthew Harbowy <hbergeronx@gmail.com> wrote:
I think there are a lot of lessons that can be taken from how Amscope upgrades their 200$ microscope to a 2500$ fluorescent microscope, without destroying the original hardware or ones eyesightFirst: use a webcam on a trinocular mount.Second: use an orange shield.The ability to use colored glass filters in the light path is the major safety and usability upgrade, and I bet that orange filters or cover slips above the sample could solve this even better. A little care and attention to your own hardware's details and you should get great results. You can also buy the upgraded fluorescent objectives: http://www.microscopenet.com/fluor-objective-fluorescent-microscopes-p-439.html?gclid=CLuf_52pobsCFW0V7AoddBcA1wMatt
On Sunday, December 8, 2013, Mega [Andreas Stuermer] wrote:> Just depends on how DIY you want to get.--
Always the same trade-off between diy efforts (and risk of destryoing the original hardware) and money^^
On Tuesday, August 10, 2010 7:49:32 AM UTC+2, Eric M wrote:Hey everybody,I'm not sure if this has already been posted, but PLoS One has got a really cool article on a $240 fluorescence microscope!
Cheers,
Eric
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