Re: [DIYbio] Re: Designing a DIY Gene Electroporator


On Tuesday, November 5, 2013 8:36:04 AM UTC-8, Cathal Garvey wrote:
In response to "is there a standard wave?",
 
no: there is no standard waveform

That said, some thoughts:
A) EP would never have taken off if there weren't a wide margin for
error

B) 
chaotic or noisy source offers the best route to general-purpose
transformation.


C) Papers are written and experiments conducted with the equipment on-hand at the time, rarely built from scratch to test a theory or a model.   Results reflect that.  So if Joe Postdoc had an HP Signal Generator which supported three types of waves (sine, triangle, pulse) then those are the tests done (or more likely, only one of those settings is used, based on lab superstition).  Even voltages in some popular papers are based on what the equipment happened to be set to, during knob-fiddling, rather than exhaustive characterization of that protocol variable.   Remember we're talking about biologists here -- papers don't measure yield, and rarely test models.   So basically, voltage, power, wave shape, etc, has not been characterized yet.   Success of noisy sources would seem imply this too.  Throw a bunch of EMF at bio, some levels succeed.   Also characterization is a painful process if each iteration takes 5 days to complete and also includes a bunch of manual steps with potential for human error at each one.    


Build my voltage step-up circuit,  http://88proof.com/synthetic_biology/blog/archives/303
control it with my high-voltage digital control board, http://88proof.com/synthetic_biology/blog/archives/338
and zap something.
(Note I didn't say "someone".) 



## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
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