Re: [DIYbio] Re: Which Antibiotics can be autoclaved

oh man. I miss sci-hub^^

On Fri, Dec 6, 2013 at 1:14 PM, Andreas Stuermer <masterstorm123@gmail.com> wrote:

Thanks, very ineresting!!

Kanamycin heat resistant? Didn't hear of that before. Activity remaining after autoclaving 98% ?

On Fri, Dec 6, 2013 at 10:47 AM, Cuong Le <cuonghieule@gmail.com> wrote:

I know this is late, but I was looking for thermal degradation data myself and after I found it I figured I would post it here for anyone else that stumbles on this thread:

Heat stability of the antimicrobial activity of sixty-two antibacterial agents

J. Antimicrob. Chemother. (1995) 35 (1): 149-154.

doi: 10.1093/jac/35.1.149

Ampicillin and tetracyclin are temperature sensitive; kanamycin is heat stable. They did not include streptomycin; however, US patent 2473339 says: "prior to the present invention it was generally known that it was impossible to autoclave solutions of streptomycin and that such solutions were injured by temperatures materially above 75° Fahrenheit" so yes/no for streptomycin but you might not be able to prepare your solutions in the way that they are making them.

On Monday, 31 December 2012 01:11:51 UTC-8, Mega [Andreas Stuermer] wrote:

Hi everybody, 

for our GFP - plant project at the AEC we will need Kanamycin, Tetracyclin and Streptomycin. 

AFAIK, you can autoclave Kanamycin (but never ever ever Ampicillin! ;) ). What about Tetracyclin and Streptomycin, will they degrade @ 121°C and 2 bars? 

Best, 

Andreas

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