Re: [DIYbio] Cloning of shine-dalgarno or kozak to CDS sans scar/gap/space

That surely is the best bet for small genes.

How about this: Use a restriction site that contains ATG? But you would also need PCR to introduce it.

Or a nice restriction site in front of the gene? So your primer is nnnnnggtaccATGnnnnnnn? The a 3 bp before atg seems to be the most important part. gccaccATG is consensus, you might get nice expression levels with GgtACCATG also?

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