[DIYbio] large dna extraction

I never worked with large DNA (2-3 Mbase) before but I have a project
that required me to. I extracted the DNA from a gram positive bacteria
using invitrogen charge-switch magnetic beads kit. What I got on the
1% gel was a band around 15kb and a band way up in the wells. So it
might be that the 15kb band is the plasmid and the other band is gDNA.

Now, I'm running the sample thru a 0.5% agarose gel at 50V. I'm
planning on cutting out the plasmid and purifiying it with one of my
qiagen gel extraction kits. But, for the large gDNA, I don't have a
kit that can handle it. They usually max at 50kb.

How would you guys approach this? Ideas?

Thanks

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAAhF0RK9BxctFB4B-hAG%3DU4sgS3vMEvvUfFbDda9z6X_b1SasA%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.