Re: [DIYbio] large dna extraction

Molecular Cloning by Sambrook lists a few methods for recovering
gel-embedded DNA. Throw the gel fragment into a piece of dialysis
tubing, let it diffuse out, roto-vap to concentrate. Or you could
electrophorese the DNA out, 'run it past the edge', then pluck out the
gel and roto-vap to concentrate.

On Mon, Feb 24, 2014 at 9:56 AM, Jeswin <phillyj101@gmail.com> wrote:
> I never worked with large DNA (2-3 Mbase) before but I have a project
> that required me to. I extracted the DNA from a gram positive bacteria
> using invitrogen charge-switch magnetic beads kit. What I got on the
> 1% gel was a band around 15kb and a band way up in the wells. So it
> might be that the 15kb band is the plasmid and the other band is gDNA.
>
> Now, I'm running the sample thru a 0.5% agarose gel at 50V. I'm
> planning on cutting out the plasmid and purifiying it with one of my
> qiagen gel extraction kits. But, for the large gDNA, I don't have a
> kit that can handle it. They usually max at 50kb.
>
> How would you guys approach this? Ideas?
>
> Thanks
>
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--
-Nathan

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