Just throw lots of DNA at the spin column. Your recovery from the silica might be less than 50% but it will be >0. Or you can use the spin columns from Zymo Clean and Concentrate with qiagen gel extraction. Put the denaturing buffer through the column just as with Qiagen, then use the Zymo wash buffer. This gives a cleaner DNA spec curve on nanodrop than the Qiagen kit.
-Rob
On Mon, Feb 24, 2014 at 11:56 AM, Jeswin <phillyj101@gmail.com> wrote:
I never worked with large DNA (2-3 Mbase) before but I have a project
that required me to. I extracted the DNA from a gram positive bacteria
using invitrogen charge-switch magnetic beads kit. What I got on the
1% gel was a band around 15kb and a band way up in the wells. So it
might be that the 15kb band is the plasmid and the other band is gDNA.
Now, I'm running the sample thru a 0.5% agarose gel at 50V. I'm
planning on cutting out the plasmid and purifiying it with one of my
qiagen gel extraction kits. But, for the large gDNA, I don't have a
kit that can handle it. They usually max at 50kb.
How would you guys approach this? Ideas?
Thanks
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