Contact the bacillus genetic stock center. The professor leading it is an awesome guy and will probably send you some normal Bacillus for free if you ask.
On Saturday, February 22, 2014 10:27:45 AM UTC-8, Advait Patil wrote:
-- On Saturday, February 22, 2014 10:27:45 AM UTC-8, Advait Patil wrote:
Hi,I am doing a science fair project on how salinity affects the amount of oil degraded by the bacteria Bacillus subtilis.I have the freeze-dried culture B. subtilis from Carolina scientific, I have rehydrated it and incubated it in a tube in nutrient broth at 30 degrees Celsius for 12 hours, but there is no growth.Do you think there will be any growth? The rehydration/incubation period for the bacteria is 24-48 hours, and the bacteria are not showing any signs of growth. There are only fifteen days left for my experiment.If not, does anyone have B. subtilis culture that I can have (not freeze dried; ready to use)? I need to start experimentation asap, so I need culture within the next two-three days. Also, does any place sell B. subtilis locally? This way I won't have to wait for shipping and I can start my experiment. I have nutrient broth, so I only need 2mL, a very small amount. This would be a great help if someone could provide culture.Thanks!
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