First you have to find the metabolic pathway that leads to vitamin C formation. Make sure to find out where it starts from. This starting substance must already be present in yeast.
-- Then gene1 turns starting substance into metabolite1.
Gene2 turns metabolite1 into metabolite2.
....
Gene6 finally turns metabolite5 into vitaminC.
Then you have a list of all genes needed and you are sure it will work like this. Then you would have to synthesize the genes (in this hypothetical example 6 genes, each of which likely has 1200 base-pairs). Synthesis costs 0.30$ per base pair roughly. Have them synthesized under control of a yeast promoter plus kozak sequence (copy plus paste the sequence from a commercial yeast plasmid :D ), and terminator. If you have it synthesized right into a yeast integrative plasmid, all you have to do is put the plasmid into yeast and select for it. Very easy to design the sequence, no efforts to synthesize. You just have to pay for synthesis, and that may be ~2000-3000$....
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