Hi Erik,
we did a similar workshop few months ago during a mini maker faire. We extracted human DNA from saliva and we amplified some high variable region of human chromosomes to make DNA fingerprinting, it is easy, fast and worked well. All we used was a really slow centrifuge (1000 RPM are enough), an OpenPCR, a water boiler and gel electrophoresis apparatus.
Check our post about it and if you are interested I can give you all the needed information.
Cheers,
Alessio - DIYbio Groningen
On 7 Feb 2014, at 16:58, xox <xoxoxcom@gmail.com> wrote:
Hey everyone!I am considering ideas for an introductoy PCR/Gel workshop - what could we test for? I remember being part of such a class/workshop a bit back - we sampled our saliva and tested for some gene that some people have, I can't remember which (though it might have been the "bitter taste gene" listed below).I like using saliva and the simple premise of testing for the presence of x gene, as it simplifies the DNA extraction and the overall concept so we can focus on the tools and concepts of PCR and Gel-running.Possible topics?I thought at first of the so-called human mendelian traits, like the classic ones you learn in high school to talk about basic genetics (tongue-rolling, widow's peak, etc) though it seems many of these are generally bogus and/or don't have worthwhile gene to home in on anyway. The two I did come up with were:1. ABO Blood Type - I guess we could take a pinprick blood sample and proceed with that?2. TAS2R38 - a "bitter taste receptor" that some haveWhat are everyone thoughts? What are some good subjects for an introductory pcr/gel session, and what human genes are of interest to test? Any comments from people with ideas, who've been part of such introductory classes or have any related things to say are welcome. Shouts out from the biohackers in Vancouvererik--
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