Re: [DIYbio] Re: Gene splicing equipment?

http://diyhpl.us/wiki/diybio/faq/equipment/

https://www.wepay.com/events/gelis-beta
(which is documented here:
http://tequals0.wordpress.com/2013/07/25/an-electrophresis-system-for-diybio/?relatedposts_exclude=1209)

for 'splicing' you'll likely need a PCR thermocycler, and a
constant-temperature heat block (or hot water bath), in addition to a
gel system (including visualization setup for cutting out DNA bands)
and micropipettors, silica DNA purification spin-columns, clean
sterile nuclease-free PCR tubes, other random smallish beakers and
flasks for mixing buffers and gel solutions. Orange capped tubes are
handy too, the 50 and 25ml especially:
http://www.orangesci.com/uploads/images/tc%2044/Tubes_gamme.jpg

(even the styrofoam trays the orange-capped tubes are shipped in
become invaluable as a general tube-stand and students will fight over
who gets to use them!)

On Thu, Feb 13, 2014 at 1:42 PM, SC <stacy734@yahoo.com> wrote:
> Hi Vincent,
>
> Welcome! While many people here make their own equipment, I prefer to buy
> mine. Usually used, or last year's models.
> For basic molecular biology, here's a beginner list:
>
> An electrophoresis unit (for running DNA on gels)
> A small "personal" centrifuge.
> A source of long wave UV light. Small handheld units are not expensive.
> A pH meter, or you can substitute paper pH strips
> A refrigerator (don't use food fridge)
> A freezer (don't use food freezer)
> Pipettors
> Culture tubes or plates (I like tubes)
> Petri plates.
>
> Stuff you probably don't need right off but you might want later:
>
> A PCR machine (depends on what you want to do)
> Stirring hot plate
> autoclave
> Something to photograph the gels (gel doc system or jury rigged web cam)
> incubator
>
> Check Ebay, Amazon, Craigslist. Remember it might take a while to find all
> the pieces if you're looking for a good deal.
>
> If you let us know what types of projects you'd like to work on, we'd be
> happy to give you some more specific advice.
>
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--
-Nathan

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