Re: [DIYbio] Re: IndieBB Campaign Survey; Help Appreciated

Cool, thanks. I guess I would need to know which part of Spathiphyllum's DNA codes for 'air cleaning' and which doesn't. Is there a maxium size of base pairs that can be put into plasmids?
Another question, where do I buy restriction enzymes and ligase, and how much would that cost? Can I ship that to The Netherlands without problems?

On Saturday, 22 February 2014 18:38:29 UTC+1, Mega [Andreas Stuermer] wrote:
Hi Dirk,
In theory yes. The kit allows you to take the "air cleaning genes" and put it into the plasmid (but you need to buy the restriction enzymes and ligase yourself). if you incubate plant protoplasts with PEG and DNA it will take up the DNA.

You could do that with any other plasmid too. But this gives you the advantage of avoiding antibiotic resistances.

On the other hand, the immediate target of the kit is to learn the basics of DNA work in bacteria.

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