Re: [DIYbio] Re: Science Fair - HELP NEEDED

On Sat, Feb 22, 2014 at 3:28 PM, Koeng <koeng101@gmail.com> wrote:
> BTW i am curious why you'd freeze dry bacillus, they spore well, i mean you
> can just leave a culture out and they will spore in that

Probably just extra precaution, since it's Carolina they've got the
money to invest in a freeze drier, and if it extends shelf-life it's
likely more than worth it for them as a big company with wide a
variety of stock. So likely they use the freeze drier for the other
strains that require it, and it works for b.subtilis spores too.

The whole going around the line of phase change thing is pretty
awesome! If you're interested Ben Krasnow has made a few DIY machines,
but he's really smart (in a mathematical/engineering sense) and has
tons of tools... so it might take a well-rounded hackerspace or a good
mechanical engineer to pull this off safely. Having one of these
designs in the DIYbio toolbox would be pretty cool! They're required
for getting good resolution with bacteria in electron microscopy, and
can be used to replace VOCs in extractions (i.e. for metabolic
engineering product confirmation).
https://www.youtube.com/watch?v=2wkgBPk0DWg

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