Re: [DIYbio] Re: Student group needs help genetically modifying foods.

That's a great find Patrik! Really cool!

On Mon, Feb 10, 2014 at 4:39 PM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
> For something as simple as Vitamin C biosynthesis in yeast, chances are
> someone has already tried this. In this case, a simple google search led to
> this paper:
>
> Biosynthesis of Vitamin C by Yeast Leads to Increased Stress Resistance
>
> in which they show that you only need three genes to produce L-ascorbic acid
> (vitamin C) in yeast, although they eventually added two more to avoid a
> bottleneck in the tail end of the pathway.
>
> Engineering five genes into yeast from scratch is still beyond what can be
> done with a highschool team (and Vitamin E looks even harder). Luckily for
> you, the 2011 Johns Hopkins iGEM team already made a plasmid with the three
> key enzymes, although I'm not sure they got it to work:
>
> http://2011.igem.org/Team:Johns_Hopkins/Project/VitC
>
> Instead of Vitamin C or E, you could focus on Vitamin A (aka beta-carotene)
> instead. The same Johns Hopkins team also made a 3-gene plasmid for Vitamin
> A biosynthesis, and demonstrated that it worked in yeast (and baked a bread
> with it!):
>
> http://2011.igem.org/Team:Johns_Hopkins/Project/VitA
> http://2011.igem.org/Team:Johns_Hopkins/Vit/Results
>
> You (or one of your teachers) may be able to get the plasmid from this
> group. At that point, it would simply be a matter of transforming it into
> yeast, and testing it out - something that should be well within reach of a
> high school team. If you get that done, you can thenn modify the pathway
> further to produce any of a range of other brightly colored carotenoids,
> such as zeaxanthin or canthaxanthin.
>
> Patrik
>
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--
-Nathan

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