B.subtilis is a survivor, was your stock culture liquid or dry? One way I've been taught to preserve b.subtilis is with 'potato chip' plates, basically agar with b.subtilis growing that you leave on the lab bench (petri dish closed) until the agar gets so dry it warps and looks like a potato chip! The b.subtilis forms spores and will last years and years and years.
12 hours isn't much time for seeing growth, give it another half-day to get upset! What broth did you use? Did you try streaking some agar plates?
One thing a lot of organisms dont like is being too dilute, so if you used too much broth to begin the first culture, it could be growing super slowly.... Try less volume in the first culture.
On Feb 22, 2014 10:27 AM, "Advait Patil" <epicadvait@gmail.com> wrote:
-- --Hi,I am doing a science fair project on how salinity affects the amount of oil degraded by the bacteria Bacillus subtilis.I have the freeze-dried culture B. subtilis from Carolina scientific, I have rehydrated it and incubated it in a tube in nutrient broth at 30 degrees Celsius for 12 hours, but there is no growth.Do you think there will be any growth? The rehydration/incubation period for the bacteria is 24-48 hours, and the bacteria are not showing any signs of growth. There are only fifteen days left for my experiment.If not, does anyone have B. subtilis culture that I can have (not freeze dried; ready to use)? I need to start experimentation asap, so I need culture within the next two-three days. Also, does any place sell B. subtilis locally? This way I won't have to wait for shipping and I can start my experiment. I have nutrient broth, so I only need 2mL, a very small amount. This would be a great help if someone could provide culture.Thanks!
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