Re: [DIYbio] That Canadian company (or part of a uni) that was making a magnetic bead plasmid assembly kit

On Wed, Feb 19, 2014 at 1:05 PM, Dan <djwrister@gmail.com> wrote:
> I used it at the FBI sponsored conference at BioCurious. It worked like a charm. LaBiohackers has taught two classes using the new kit. The only downside is you really are stuck with what is in the kit, which is good for a safety perspective, but you can't really make your own poly magnetic beads or easily order them cheaply.

Dan, what do you mean by 'stuck with what is in the kit'?

Compared to the $400 genomikon kit, which I don't know how much
magnetic beads they include, these beads are less than that:
http://www.bioclone.us/DNA-RNA-conjugation-Immobilization-magnetic-beads-particle-resin-matrix.html
http://www.bangslabs.com/products/magnetic_microspheres
https://www.neb.com/products/s1419-oligo-dt25-magnetic-beads

You can get the oligo dT already attached, and require that your
starting biobrick or whatever the kids are calling DNA elements these
days has a matching stretch of As... or you can get the coupling kits
and attach your first strand directly to the bead:
http://www.idtdna.com/pages/docs/technical-reports/strategies-for-attaching-oligonucleotides-to-solid-supports.pdf?sfvrsn=4

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